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cTnI from the sample will become labeled by having an ALP-conjugated antibody and is particularly captured on to the sensor area throughout the incubation stage.[49] Wash fluid containing enzyme substrate is then applied to take away unbound substances, and simultaneously, ALP certain to the antigen-antibody complicated reacts, releasing an electrochemically detectable solution. The created amperometric sign is instantly proportional to your cTnI from the sample.[fifty]
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